Green fluorescent protein – GFP – is a favourite trick among biologists for looking closely at cells.
Originally found in the jellyfish Aequorea Victoria, the protein glows bright green under precise wavelengths of UV light.
For several decades, scientists have been adding the GFP jellyfish gene to genetically modified animals, plants, and other organisms, to check where they’re expressing genes.
It’s also a useful tag to investigate microscopic processes in cells.
But, while small and non-disruptive, GFP isn’t always the perfect tag: it often needs very sensitive imaging equipment to see the glowing protein, like fluorescence microscopy.
This has spurred researchers to look for and make similar proteins that dodge GFP’s limitations.
One, described in a paper in Horticulture Research, lets us see the protein glowing with a black light and the naked eye.
The protein, charmingly called eYGFPuv, is a modified form of a GFP-like protein, developed specifically for use in plants.
The researchers were able to show that the gene for eYGFPuv could be adopted by four different plant species: the small herbaceous Arabadopsis and tobacco, and the large woody citrus and poplar.
Chlorophyll goes red under UV light, so plants that without this genetic modification showed up pink in a black light.
But the plants that were given the gene for eYGFPuv glowed a bright shade of green instead.
This technique works at both the macroscale, over the whole plant, and the microscale – in seedlings or petri dish cells.
It’s also obvious in dim light – unlike original GFP, which usually needs complete darkness in order to see the glow.
“These features suggest that eYGFPuv has a wide range of applications in plant science research,” write the researchers in their paper.
Originally published by Cosmos as Making a pink plant glow bright green with genetic modification
Ellen Phiddian
Ellen Phiddian is a science journalist at Cosmos. She has a BSc (Honours) in chemistry and science communication, and an MSc in science communication, both from the Australian National University.
Read science facts, not fiction...
There’s never been a more important time to explain the facts, cherish evidence-based knowledge and to showcase the latest scientific, technological and engineering breakthroughs. Cosmos is published by The Royal Institution of Australia, a charity dedicated to connecting people with the world of science. Financial contributions, however big or small, help us provide access to trusted science information at a time when the world needs it most. Please support us by making a donation or purchasing a subscription today.