A human cell, inflated


New method allows better imaging without distortion.


The microtubules forming the cytoskeleton of a single human cell are visible in this inflated specimen

The microtubules forming the cytoskeleton of a single human cell are visible in this inflated specimen.

UNIGE

There are two ways to get a closer look at something very small: build a better microscope or make the object larger.

The problem with the second option – especially if the object of attention is a biological cell, and thus full of liquid – is that enlargement and deformation tend to go hand in hand. The object becomes easier to see, but it also becomes damaged in the process.

Until now. In a paper published in the journal Nature Methods, a team led by Virginie Hamel at the University of Geneva in Switzerland reveal a new approach called Ultrastructure Expansion Microscopy (U-ExM), which permits biological samples to be inflated without the need for prior chemical fixation.

“Cells gradually expand and their components separate from each other while enlarging,” says co-author Davide Gambarotto.

“The architecture of the various elements is preserved and it becomes possible to observe them with a resolution hitherto unattained in optical microscopy.”

  1. http://dx.doi.org/10.1038/s41592-018-0238-1
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